Revision as of 10:54, 9 August 2010

Transformation of pVeg and lacI

There are colonies on all 3 plates for pVeg.
For lacI, there is only one colony on one of the normal plates and more on the concentrated one.

For pVeg, we will set up an overnight broth culture from the colonies we got on the plates for plasmid extraction
To show that we have sucessfully transformed from lacI, we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.

To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

Please refer to 10.08.10 for result.

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 ==Results/Aims==
-* pVeg was successfully transformed - From this we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]]
+* There are colonies on all 3 plates for pVeg.
-* ''lacI'' - To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
+* For ''lacI'', there is only one colony on one of the normal plates and more on the concentrated one.
+==Discussion==
+* For pVeg, we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] from the colonies we got on the plates for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]]
+* To show that we have sucessfully transformed from ''lacI'', we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
 =Transformation of lacI=