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Team:Newcastle/9 August 2010
From 2010.igem.org
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==Materials and Protocol== | ==Materials and Protocol== | ||
| - | Please refer to [[TeamNewcastleTransformation_of_E. | + | Please refer to [[TeamNewcastleTransformation_of_E._coli|Transformation of E.coli]]. |
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Revision as of 10:18, 9 August 2010
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Contents |
Transformation
Results/Aims
- pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
Transformation of lacI
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Materials and Protocol
Please refer to Transformation of E.coli.
   
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