"
Team:Newcastle/16 July 2010
From 2010.igem.org
(Difference between revisions)
(→Results) |
|||
| Line 17: | Line 17: | ||
==Results== | ==Results== | ||
| - | The single digests (lanes 1-7) and the double digests (lanes (9-15) are separated by the molecular marker (lane 8). | + | *The single digests (lanes 1-7) and the double digests (lanes (9-15) are separated by the molecular marker (lane 8). |
[[Image:Newcastle LacI BioBrick Construction Gel.png|700px|centre]] | [[Image:Newcastle LacI BioBrick Construction Gel.png|700px|centre]] | ||
Revision as of 07:50, 9 August 2010
| |||||||||||||
| |||||||||||||
Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
- The aim of today's experiment is to screen for the lacI insert. This could be done using PCR but that would require specific primers and is not as reliable.
Protocol
- Miniprep - the miniprep protocol was followed.
- Restriction digests - the restriction digests protocol was followed to allow the length of the DNA to be checked using single and double digest.
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis - the protocol for gel electrophoresis was followed.
- Set up liquid broth culture in LB. The protocol for growing an overnight culture was followed.
Results
- The single digests (lanes 1-7) and the double digests (lanes (9-15) are separated by the molecular marker (lane 8).
Inference
   
|
    
|
