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Team:Newcastle/14 July 2010
From 2010.igem.org
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*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | ||
| - | Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests. | + | *Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests. |
Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday) | Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday) | ||
Revision as of 14:03, 6 August 2010
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LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
- Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests.
Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday)
Protocol
we followed Wendy's cards/Phil's lab-book
   
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