Gel electrophoresis

Materials

1. 50X TAE buffer
   • Make up 1 liter of 50X TAE buffer with the following:
   • 242g of TRIS base
   • 57.1 ml of acetic acid
   • 100 ml of 0.5 M of EDTA (pH 8.0)
   • Top up to 1 liter with water
2. SafeView
3. Agarose
4. DNA ladder
5. Eppendorf
6. Gel making tank
7. Gel running tank

Procedure

1. Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview dye.
2. Wait for 30 min to allow the gel to harden.
3. Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerged.
4. Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder is used for analysing DNA.
5. Loading buffer is then added together with the sample before loading onto the gel matrix.
6. Run gel at 90V until the desired separation is achieved and visualize using the gelDoc.

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