"
Team:Newcastle/Transformation of B. subtilis
From 2010.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
=Transformation of ''Bacillus subtilis''= | =Transformation of ''Bacillus subtilis''= | ||
| + | |||
| + | ==Materials required== | ||
| + | # ''Bacillus subtilis'' 168 | ||
| + | # 10 µl of DNA | ||
| + | # SMM medium (1 liter) | ||
| + | #* 2.0 g of ammonium sulphate | ||
| + | #* 14.0g of dipotassium hydrogen phosphate | ||
| + | #* 6.0g of potassium dihydrogen phosphate | ||
| + | #* 1.0g of sodium citrate dehydrate | ||
| + | #* 0.2g of magnesium sulphate | ||
| + | #* Top up the rest of the medium with water | ||
| + | |||
| + | |||
| + | |||
| + | |||
Revision as of 12:06, 5 August 2010
| |||||||||||||
| |||||||||||||
Transformation of Bacillus subtilis
Materials required
- Bacillus subtilis 168
- 10 µl of DNA
- SMM medium (1 liter)
- 2.0 g of ammonium sulphate
- 14.0g of dipotassium hydrogen phosphate
- 6.0g of potassium dihydrogen phosphate
- 1.0g of sodium citrate dehydrate
- 0.2g of magnesium sulphate
- Top up the rest of the medium with water
Go back to our Protocol List
   
|
    
|
