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Team:Newcastle/16 July 2010
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | + | = Screening for lacI insert = | |
| - | *#Miniprep - | + | |
| - | *#Restriction digests | + | == Aim == |
| + | The aim of this part of this experiment is to screen for the lacI insert. | ||
| + | |||
| + | == Protocol == | ||
| + | *#Miniprep - the [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed. | ||
| + | *#Restriction digests - the [[Team:Newcastle/Restriction_digests|restriction digests]] protocol was followed to allow the length of the DNA to be checked using single and double digest. | ||
*#*Single digest with Pst1 | *#*Single digest with Pst1 | ||
*#*double digests with Pst1 and Xba1 | *#*double digests with Pst1 and Xba1 | ||
| - | *#Gel electrophoresis | + | *#Gel electrophoresis - the [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocol was followed. |
| + | |||
| + | Inference | ||
*Set up liquid broth culture in LB | *Set up liquid broth culture in LB | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 10:50, 5 August 2010
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Screening for lacI insert
Aim
The aim of this part of this experiment is to screen for the lacI insert.
Protocol
- Miniprep - the miniprep protocol was followed.
- Restriction digests - the restriction digests protocol was followed to allow the length of the DNA to be checked using single and double digest.
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis - the gel electrophoresis protocol was followed.
Inference
- Set up liquid broth culture in LB
   
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