Team:Newcastle/4 August 2010

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===PCR===
===PCR===
-
 
+
{|border=1
 +
|-
 +
!'''pSB1C3
 +
(No. 1)'''
 +
!'''pSB1C3
 +
(No. 2)'''
 +
!'''pSB1C3
 +
(No. 3)'''
 +
!'''pSB1C3
 +
(No. 4)'''
 +
!'''lacI
 +
(No. 1)'''
 +
!'''lacI
 +
(No. 2)'''
 +
!'''Double terminator !'''Double terminator
 +
(No. 1)'''
 +
!'''Double terminator
 +
(No. 2)'''
 +
|-
 +
|44.0 µl/ml
 +
|19.9 µl/ml
 +
|25.0 µl/ml
 +
|30.8 µl/ml
 +
|10.0 µl/ml
 +
|44.2 µl/ml
 +
|9.2 µl/ml
 +
|39.7 µl/ml
 +
|}
==Gibson assembly==
==Gibson assembly==
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 08:48, 5 August 2010

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Contents

Cloning the rocF BioBrick

Aim

The aim of today's experiment is to amplify 6 different fragments for the construction of rocF BioBrick with the help of 6 different Phusion PCR.

Materials and Protocol

Please refer to PCR for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below:

PCR

pSB1C3

(No. 1)

pSB1C3

(No. 2)

pSB1C3

(No. 3)

pSB1C3

(No. 4)

lacI

(No. 1)

lacI

(No. 2)

Double terminator !Double terminator

(No. 1)

Double terminator

(No. 2)

44.0 µl/ml 19.9 µl/ml 25.0 µl/ml 30.8 µl/ml 10.0 µl/ml 44.2 µl/ml 9.2 µl/ml 39.7 µl/ml

Gibson assembly

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
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