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Team:Newcastle/4 August 2010
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | + | =Cloning the ''rocF'' BioBrick= | |
==Aim== | ==Aim== | ||
The aim of today's experiment is to amplify 6 different fragments for the construction of [[Team:Newcastle/Urease|''rocF'' BioBrick]] with the help of 6 different Phusion PCR. | The aim of today's experiment is to amplify 6 different fragments for the construction of [[Team:Newcastle/Urease|''rocF'' BioBrick]] with the help of 6 different Phusion PCR. | ||
| - | ==PCR== | + | ==Materials and Protocol== |
| + | Please refer to [[Team:Newcastle/PCR| PCR]] for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below: | ||
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| + | ===PCR=== | ||
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==Gibson assembly== | ==Gibson assembly== | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 08:47, 5 August 2010
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Contents |
Cloning the rocF BioBrick
Aim
The aim of today's experiment is to amplify 6 different fragments for the construction of rocF BioBrick with the help of 6 different Phusion PCR.
Materials and Protocol
Please refer to PCR for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below:
PCR
Gibson assembly
   
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