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Team:Newcastle/15 July 2010
From 2010.igem.org
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
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Screening for lacI insert: | Screening for lacI insert: | ||
#(Miniprep) | #(Miniprep) | ||
| - | #Restriction digests | + | #Restriction digests - the [[Team:Newcastle/Restriction_digests| restriction digest]] protocol was followed. |
#*Single digest with Pst1 | #*Single digest with Pst1 | ||
#*double digests with Pst1 and Xba1 | #*double digests with Pst1 and Xba1 | ||
#Gel electrophoresis | #Gel electrophoresis | ||
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Set up liquid broth culture in LB | Set up liquid broth culture in LB | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 15:52, 5 August 2010
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Screening for lacI insert:
- (Miniprep)
- Restriction digests - the restriction digest protocol was followed.
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis
Set up liquid broth culture in LB
   
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