Team:Newcastle/16 July 2010

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==Summary==
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*Screening for lacI insert:
*Screening for lacI insert:
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*#Miniprep
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*#Miniprep - The [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed.
*#Restriction digests
*#Restriction digests
*#*Single digest with Pst1  
*#*Single digest with Pst1  

Revision as of 10:37, 5 August 2010

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  • Screening for lacI insert:
    1. Miniprep - The miniprep protocol was followed.
    2. Restriction digests
      • Single digest with Pst1
      • double digests with Pst1 and Xba1
    3. Gel electrophoresis
  • Set up liquid broth culture in LB
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