Team:Newcastle/16 July 2010
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*Screening for lacI insert: | *Screening for lacI insert: | ||
- | *#Miniprep | + | *#Miniprep - The [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed. |
*#Restriction digests | *#Restriction digests | ||
*#*Single digest with Pst1 | *#*Single digest with Pst1 |
Revision as of 10:37, 5 August 2010
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- Screening for lacI insert:
- Miniprep - The miniprep protocol was followed.
- Restriction digests
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis
- Set up liquid broth culture in LB