Team:Calgary/30 July 2010

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[[Image:07.30.2010-ChriscpxPgel2.jpg|thumb|400px|Chris's second gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50&deg;C to 56&deg;C and at a MgCl<sub>2</sub> concentration of 2.0 &micro;M.]]
[[Image:07.30.2010-ChriscpxPgel2.jpg|thumb|400px|Chris's second gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50&deg;C to 56&deg;C and at a MgCl<sub>2</sub> concentration of 2.0 &micro;M.]]
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<u> Himika </u>
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Today there was a meeting which updated updates all the team members about each other. I also kept looking into MATLAB tutorials and tried out some of the tutorials. I also read some of the papers about inclusion bodies. This will be used to model of the project. The sequence data of the I0500-B0034 came back today and it was successful it seems. So I have decided to go forth with the construct and add on more parts next week.
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Revision as of 22:36, 30 July 2010

Friday July 30, 2010

Chris's first gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl2 concentration of 1.5 µM.
Chris's second gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl2 concentration of 2.0 µM.


Himika

Today there was a meeting which updated updates all the team members about each other. I also kept looking into MATLAB tutorials and tried out some of the tutorials. I also read some of the papers about inclusion bodies. This will be used to model of the project. The sequence data of the I0500-B0034 came back today and it was successful it seems. So I have decided to go forth with the construct and add on more parts next week.

No notebook page exists for this date. Sorry!