Team:Newcastle/Filamentous Cells
From 2010.igem.org
(Difference between revisions)
RachelBoyd (Talk | contribs) (→YneA) |
Swoodhouse (Talk | contribs) |
||
Line 1: | Line 1: | ||
{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
+ | Molecular tweezer tensile strength test. | ||
+ | |||
=Filamentous Cells= | =Filamentous Cells= | ||
==Filamentous cells genes list== | ==Filamentous cells genes list== |
Revision as of 17:55, 3 August 2010
|
Molecular tweezer tensile strength test.
Contents |
Filamentous Cells
Filamentous cells genes list
- yneA (Transcribed with yneB, ynzC, is an analogue of sulA in E.coli) * our biobrick is designed to over express this gene reducing cell division possibly by inhibiting FtsZ ring formation or constriction.
- dinR (Homologue of lexA in E.coli transcribed in the opposite direction)
- ftsZ (Involved in the recruitment of other proteins to the divisisome for cytokinesis, strangely over expression results in disruption of Zring formation as well as reduced expression)
- secA (Involved in the secretion of extracellular proteins and the insertion of transmembrane proteins)
- recA (Involved in SOS response removing the repressor DinR (LexA))
- wpr and epr produce extracellular proteases that cleave the signal peptide/transmembrane domain of YneA
- ezr produces a protein which sequesters FtsZ monomer by binding its C terminal domain and also inhibits GTP binding; however overexpression does not result in filamentation.
- min C,D ,J and divIVA prevent polar cell division .
- Positive regulators of FtsZ: ftsA, zapA, zipA, ftsL and divIC
- Inhibitors of Daughter cell separation: lytC,D,E,F and cwlS *Chains rather than filaments, yneA is also reported to increase the time spent in chains well into the stationary phase of bacterial growth.
YneA
Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.
yneA cloning strategy
Media:yneA cloning strategy.pdf