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Team:Newcastle/Growing an overnight cultures
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# 5ml of LB broth is pipetted into the universal tube. | # 5ml of LB broth is pipetted into the universal tube. | ||
| - | # The flame loop is used to pick a colony. | + | # The flame loop is used to pick a colony present on the agar plate. |
# The colony is then mixed into the LB Broth in the universal tube. | # The colony is then mixed into the LB Broth in the universal tube. | ||
# The culture is left overnight at 37°C (''E.coli'', ''B.subtilis'' lower temp) | # The culture is left overnight at 37°C (''E.coli'', ''B.subtilis'' lower temp) | ||
Revision as of 10:12, 30 July 2010
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Growing an Overnight Culture
Materials Required
- LB Broth
- Colonies of bacteria
- Flame loop
- Universal tube
- Pipette
Procedures
- 5ml of LB broth is pipetted into the universal tube.
- The flame loop is used to pick a colony present on the agar plate.
- The colony is then mixed into the LB Broth in the universal tube.
- The culture is left overnight at 37°C (E.coli, B.subtilis lower temp)
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