"
Team:Newcastle/Arginine test
From 2010.igem.org
(Difference between revisions)
Shethharsh08 (Talk | contribs) |
|||
| Line 22: | Line 22: | ||
* Incubate the culture at 37° C with shaking. | * Incubate the culture at 37° C with shaking. | ||
* Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick. | * Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick. | ||
| + | |||
| + | |||
| + | '''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 09:16, 30 July 2010
| |||||||||||||
| |||||||||||||
Arginine Test
Materials Required
- Plate consisting of Bacillus subtilis 168 colonies.
- Flame (streaking) Loop
- LB media consisting arginine and ampicillin
- Auto pipette
- Bursen Burner
- Universal Tube
Procedures
- Perform the experiment using aseptic technique.
- Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
- Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
- Control (1) - LB media
- Control (2) - LB media with 10 mM of arginine
- Control (3) - LB media plus B. subtilis 168
- Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
- Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
- Incubate the culture at 37° C with shaking.
- Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick.
Go back to our Protocol List
   
|
    
|
