Team:Harvard/color/notebook

From 2010.igem.org

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<li class="indent">BETA-OHASE 1 rxn 2: CP6, CP7</li>
<li class="indent">BETA-OHASE 1 rxn 2: CP6, CP7</li>
<li class="indent">BETA-OHASE 1 rxn 3: CP5, B</li>
<li class="indent">BETA-OHASE 1 rxn 3: CP5, B</li>
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<li>PCR purified finished reaction and ran 4 ul on a 1% agarose gel</li>
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<li>PCR purified completed reaction</li>
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<div><a href="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png" id="single_image"><img src="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png"  width="100px"/> [click to enlarge]</a></div>
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<li>Cut out backbone (top) bands from O1, O2, R1 and saved them at 4&deg;C </li></ul>
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<span id="date"><a name="07-26-2010" class="date">07-26-2010</a></span> [<a class="top" href="#notebook">top</a>]<hr/>
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<ul>
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<li>Ran 4 ul of PCR reaction on a 1% agarose gel</li>
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<div><a href="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png" id="single_image"><img src="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png"  width="100px"/> [click to enlarge]</a></div>
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<li>Bands appeared to be the correct size, so started PCR stitching process</li>
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<li>Ran two PCR reactions, one for each of LUT2 and BETA-OHASE 1</li>
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<li class="indent">Template: Rxn 1, 2, and 3 of the appropriate construct</li>
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<li class="indent">Primers: A and B for both reactions</li>
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<li>PCR purified and ran 4 ul on a 1% agarose gel</li>
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<div><a href="https://static.igem.org/mediawiki/2010/c/c9/072610pcr2.png" id="single_image"><img src="https://static.igem.org/mediawiki/2010/c/c9/072610pcr2.png" width="100px"/> [click to enlarge]</a></div>
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Revision as of 20:11, 28 July 2010



notebook

Week 6----07-23-2010
Week 707-26-201007-27-201007-28-201007-29-201007-30-2010


07-23-2010 [top]
  • Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs
  • PCR reaction on RS300 to insert recognition sites. Primers listed below
  • LUT2 rxn 1: A, CP4
  • LUT2 rxn 2: CP2, CP3
  • LUT2 rxn 3: CP1, B
  • BETA-OHASE 1 rxn 1: A, CP8
  • BETA-OHASE 1 rxn 2: CP6, CP7
  • BETA-OHASE 1 rxn 3: CP5, B
  • PCR purified completed reaction
07-26-2010 [top]
  • Bands appeared to be the correct size, so started PCR stitching process
  • Ran two PCR reactions, one for each of LUT2 and BETA-OHASE 1
  • Template: Rxn 1, 2, and 3 of the appropriate construct
  • Primers: A and B for both reactions
  • PCR purified and ran 4 ul on a 1% agarose gel

    • Retrieved from "http://2010.igem.org/Team:Harvard/color/notebook"