Team:Cambridge/References/ProjectBioluminescence/Experiments

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=Experiments=
 
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==1. Test luminescence with the Luciferase from the registry==
==1. Test luminescence with the Luciferase from the registry==
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*Transform WT E.coli with the luciferase from the registry
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* Transform WT E.coli with the luciferase from the registry.
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* Feed with D-luciferin, develop a method for measuring light output
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* Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
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* Controls - no luciferin, no inducer
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2. Test use of CHBT as a substrate  
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==2. Test use of CHBT as a substrate==
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a) D-cysteine
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*'''a) D-cysteine'''
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* test
b) L-cysteine via L-luciferin
b) L-cysteine via L-luciferin

Revision as of 15:59, 26 July 2010