Thursday August 19, 2010

Emily<u/u>

Today I made a list of what figures we will need for our presentation next week. I also read over Raida's current draft of the Ethics paper. In the lab, I miniprepped last night's Overnight cultures of ibpAB-FsxA-T7. I then set up a verification digest of this piece of DNA with EcoRI and the EcoRI Buffer from NEB. I made overnight cultures of the psB2K3 vector as well as ibpAB-FsxA-T7. diluted the new malE-BBK-R* primer. Dev then helped me set up two reactions in order to amplify malE, malE31, malESSdel and malE31SSdel from plasmid DNA. We left this running overnight. Finally, I inoculated LB broth with my overnight cultures of I0500-I13504 for the Arabinose Induction assay. I finihsed making competant cells of the degP and CpxP strains from the Raivio Lab as well as the cells with our sequenced cpxR circuit. These competant cells are for testing purposes. Finally, Chris and I worked on purifying some PCR product of cpxP from a couple of weeks ago.