| Biobrick name |
Description |
Creation date |
Strain |
Vector |
Quality control |
Length |
Store |
|
<partinfo>BBa_E2050</partinfo>
|
mOrange
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB2K3</partinfo>
|
Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
|
744 bp | BioBricks 2010 |
|
<partinfo>BBa_K165018</partinfo>
|
NLS-ADH1 terminator (Silver Standard 5' and 3')
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
|
253 bp | BioBricks 2010 |
|
<partinfo>BBa_K165037</partinfo>
|
tef2 promoter
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (MIT)
|
403 bp | BioBricks 2010 |
|
<partinfo>BBa_P1004</partinfo>
|
Chloramphenicol resistence
cassette
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A1</partinfo>
|
Seq ok(MIT 2009); length ok
|
769 bp | BioBricks 2010 |
|
<partinfo>BBa_J61001</partinfo>
|
R6K origin
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok (MIT)
|
406 bp | BioBricks 2010 |
|
<partinfo>BBa_K081008</partinfo>
|
RBS-luxI
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok (MIT); length ok
|
664 bp | BioBricks 2010 |
|
<partinfo>BBa_K125500</partinfo>
|
GFP fusion brick (Silver Standard 5', Knight Standard 3')
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
|
718 bp | BioBricks 2010 |
|
<partinfo>BBa_J72007</partinfo>
|
BamHI methyltransferase encoding CRIM plasmid
|
01/07/2010
|
E. coli ???
|
HC Cm resistance
|
no plasmid in the clone
For more informations click here
|
| BioBricks 2010 |
|
<partinfo>BBa_J72008</partinfo>
|
phi80 integration helper plasmid pInt80-649
|
01/07/2010
|
E. coli ???
|
Plasmid (Amp 50 ug/ml resistance)
|
Length ok
|
6335 bp | BioBricks 2010 |
|
<partinfo>BBa_J72013</partinfo>
|
BglII methyltransferase encoding CRIM plasmid
|
01/07/2010
|
E. coli ???
|
HC Cm resistance
|
No plasmid in the clone
|
|
BioBricks 2010 |
| |
| Ligation name |
Description |
Creation date |
Strain |
Vector |
Quality control |
Length |
Store |
|
I0-1
|
<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I0
(it was I0-2)
|
<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (BMR); length ok
|
853 bp | Ligations 2010 |
|
I1-1
(not used)
|
<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I1
(it was I1-2)
|
<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
|
1029 bp | Ligations 2010 |
|
I2
(it was I2-1)
|
<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (BMR); Cm resistance ok; length ok.
|
906 bp | Ligations 2010 |
|
I2-2
(not used)
|
<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Not sequenced; Cm resistance ok
|
| Ligations 2010 |
|
I3
(it was I3-1)
|
<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
|
801 bp | Ligations 2010 |
|
I3-2
|
<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I4-1
(not used)
|
<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Not sequenced; length ok
|
| Ligations 2010 |
|
I4
(it was I4-2)
|
<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (BMR); length ok
|
1439 bp | Ligations 2010 |
|
I5
(it was I5-1)
|
I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok (BMR); Cm resistance ok; length ok
|
1320 bp | Ligations 2010 |
|
I6
(it was I6-2)
|
I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
|
2754 bp | Ligations 2010 |
|
I6-3
(not used)
|
I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
Not sequenced
|
| Ligations 2010 |
|
<partinfo>BBa_K208001</partinfo> (stab-1)
|
phasin phaP
(Silver Standard 5' and 3') |
24/06/2010
|
E. coli ???
|
<partinfo>pSB3K3</partinfo>
|
Seq ok (BMR); length ok
|
579 bp | BioBricks 2010 |
|
<partinfo>BBa_K208001</partinfo> (stab-2)
|
phasin phaP
(Silver Standard 5' and 3') |
24/06/2010
|
E. coli ???
|
<partinfo>pSB3K3</partinfo>
|
Seq ok (BMR); length ok
|
579 bp | BioBricks 2010 |
|
I7-1
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I7-2
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I7
(it was I7-3)
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I7-5
(not used)
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I8-1
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I8-2
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I8-4
(not used)
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I8
(it was I8-5)
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I9
(it was I9-1)
|
<partinfo>BBa_J23111</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I9-2
(not used)
|
<partinfo>BBa_J23111</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I10
(it was I10-1)
|
<partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I10-2
(not used)
|
<partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I11-1
|
<partinfo>BBa_J23101</partinfo> (S-P)
+
I6 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
|
I11-2
|
<partinfo>BBa_J23101</partinfo> (S-P)
+
I6 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
|
I12-1
(not used)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I6 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I12
(it was I12-2)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I6 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Seq ok (BMR), length ok
|
2795 bp |
Ligations 2010 |
|
I13-1
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I6 (X-P)-->
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
|
I13-2
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I6 (X-P)-->
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
|
I14
(it was I14-1)
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Seq ok (BMR), length ok
|
|
Ligations 2010 |
|
I14-2
(not used)
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
|
Ligations 2010 |
|
I15-1
|
<partinfo>BBa_J23110</partinfo> (S-S)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
|
I15-2
|
<partinfo>BBa_J23110</partinfo> (S-S)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
|
I16
(it was I16-1)
|
<partinfo>BBa_J23116</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Seq ok (BMR), length ok
|
|
Ligations 2010 |
|
I16-2
(not used)
|
<partinfo>BBa_J23116</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Not sequenced, length ok
|
|
Ligations 2010 |
|
I17
(it was I17-1)
|
<partinfo>BBa_J23101</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
|
Seq ok (BMR), length ok
|
| Ligations 2010 |
|
I17-2
(not used)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
<Not sequenced, length ok>
|
|
Ligations 2010 |
|
I18
(it was I18-1)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Seq ok (BMR), length ok
|
|
Ligations 2010 |
|
I18-2
(not used)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
<Not sequenced, length ok>
|
|
Ligations 2010 |
|
I19
(it was I19-1)
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
Seq ok (BMR), length ok
|
|
Ligations 2010 |
|
I19-2
(not used)
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
<Not sequenced, length ok>
|
|
Ligations 2010 |
|
I74C5-1
(not used)
|
I7 (E-P)
+
<partinfo>pSB4C5</partinfo>(E-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Not sequenced, length ok
|
| Ligations 2010 |
|
I74C5
(it was I74C5-2)
|
I7 (E-P)
+
<partinfo>pSB4C5</partinfo>(E-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Seq ok (BMR), length ok
|
|
Ligations 2010 |
|
I84C5-1
(not used)
|
I8 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Not sequenced, length ok
|
|
Ligations 2010 |
|
I84C5
(it was I84C5-2)
|
I8 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Seq ok (BMR), length ok
|
|
Ligations 2010 |
|
I104C5-2
|
I10 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
16/07/2010
|
E. coli DH5-alpha
|
|
Wrong clone
|
| Ligations 2010 |
| |
| Strain name |
Description |
Arrival date |
Infos |
Resistance |
Quality control |
Notes |
Store |
|
MG1655(Seq)
(MG)
|
E. coli wild type strain fully sequenced
|
28/06/2010
|
link
|
- |
|
Genome fully sequenced |
Strains 2010 |
|
MC1061
(MC)
|
E. coli strain
|
28/06/2010
|
link
|
- |
|
Strain used by JC Anderson et al. for integration |
Strains 2010 |
|
BW25141
(BW1)
|
E. coli pir+ strain
|
28/06/2010
|
link
|
- |
Actually replicates the conditional replication origin R6K |
LC pir |
Strains 2010 |
|
BW25142
(BW2)
|
E. coli pir116 strain
|
28/06/2010
|
link
|
- |
Actually replicates the conditional replication origin R6K |
HC pir |
Strains 2010 |
|
BW23473
|
E. coli pir+ strain
|
28/06/2010
|
link
|
- |
|
LC pir |
Strains 2010 |
|
BW23474
|
E. coli pir116 strain
|
28/06/2010
|
link
|
- |
Actually replicates the conditional replication origin R6K |
HC pir |
Strains 2010 |
|
BW5328/pAH123
|
E. coli strain
|
28/06/2010
|
link
|
Amp 50 ug/ml |
Plasmid length ok (after digestion with SpeI) |
Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C
|
Strains 2010 |
|
BT340
|
E. coli strain
|
28/06/2010
|
link
|
Amp 100 ug/ml and Cm 34 ug/ml |
Plasmid relative molecular weight ok (after gel run of the undigested plasmid) |
Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C
|
Strains 2010 |
|
DSM15372
|
E. coli DMS15372 strain
|
08/07/2010
|
link
|
Amp 100 ug/ml |
Length ok (after digestion with EcoRI) |
Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)
|
Strains 2010 |
"
