"

From 2010.igem.org

Revision as of 19:15, 19 July 2010 (view source) Anisha (Talk | contribs) (New page: LB media * 10 g LB broth * 500 mL DI water autoclave to sterilize LB media with agar * 10 g LB broth * 7.5 g agar * 500 mL DI water autoclave to sterilize add a...) ← Older edit		Revision as of 19:21, 19 July 2010 (view source) Anisha (Talk | contribs) Newer edit →
Line 1:		Line 1:
-	LB media	+	LB Media (RT)
		+	* 10g Tryptone
		+	* 10g NaCl
		+	* 5g Yeast Extract
		+	* 1L Deionized Water
		+
-	* 10 g LB broth	+	SOB Media (RT)
-	* 500 mL DI water	+	* 20g Tryptone
		+	* 5g Yeast Extract
		+	* 0.5g NaCl
		+	* 0.2g KCl
		+	* 1L Deionized Water
-	autoclave to sterilize
-	LB media with agar	+	SOC Media (4 degrees Celsius)
		+	* Add the following per ml SOB:
		+	- 4ul MgCl2 Solution (100)
		+	- 8ul Glucose Solution (200)
-	* 10 g LB broth
-	* 7.5 g agar
-	* 500 mL DI water
-	autoclave to sterilize	+	Glucose Stock Solution (4 degrees Celsius)
-		+	* 5g glucose
-	add antibiotics after autoclaving when warm to touch (about 50°C)	+	* 10ml Deionized Water
-		+
-	pour plates when warm to the touch (about 50°C)	+
-		+
-		+
-	Ampicillin Antibiotic Stock Solution (100 mg/mL)	+
-		+
-	* 1 g of ampicillin	+
-	* 5 mL of DI water	+
-	* 5 mL of ethanol	+
-		+
-	filter sterilize and make 1 mL aliquotes to store in the -20°C freezer	+
-		+
-		+
-	Kanamycin Antibiotic Stock Solution (50 mg/mL)	+
-		+
-	* 250 mg of kanamycin	+
-	* 5 mL of DI water	+
-		+
-	filter sterilize and make 1 mL aliquots to store in the -20°C freezer	+
-		+
-		+
-	Tetracycline Antibiotic Stock Solution (50 mg/mL)	+
-		+
-	* 250 mg of tetracycline	+
-	* 2.5 mL of DI water	+
-	* 2.5 mL of ethanol	+
-		+
-	filter sterilize and make 1 mL aliquots to store in the -20°C freezer	+

---

Revision as of 19:21, 19 July 2010

LB Media (RT)

  * 10g Tryptone
  * 10g NaCl
  * 5g Yeast Extract
  * 1L Deionized Water
  

SOB Media (RT)

  * 20g Tryptone 
  * 5g Yeast Extract
  * 0.5g NaCl
  * 0.2g KCl
  * 1L Deionized Water

SOC Media (4 degrees Celsius)

  * Add the following per ml SOB:
      - 4ul MgCl2 Solution (100)
      - 8ul Glucose Solution (200)

Glucose Stock Solution (4 degrees Celsius)

  * 5g glucose
  * 10ml Deionized Water