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Team:TU Delft/30 June 2010 content
From 2010.igem.org
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| - | + | =Lab work= | |
| - | + | ==BioBrick stocks== | |
We harvested the 50 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010_content 14 BioBricks]. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. | We harvested the 50 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010_content 14 BioBricks]. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. | ||
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| - | + | ==''Pseudomonas Putida'' GPO1== | |
''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives. [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010 Absorbance measurements] | ''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives. [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010 Absorbance measurements] | ||
Revision as of 19:20, 2 August 2010
Lab work
BioBrick stocks
We harvested the 50 mL bacterial cells of the 14 BioBricks. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation.
The following plasmid concentrations were obtained:
| BioBrick | Concentration (ng/μL) |
| B0015 | 360.5 |
| B0032 | 80.4 |
| B0034 | 181.7 |
| E0040 | 66.0 |
| E0240 | 305.6 |
| E0422 | 411.0 |
| J61100 | 128.7 |
| J61101 | 167.7 |
| J61107 | 195.9 |
| J61117 | 62.7 |
| J61127 | 33.8 |
| R0010 | 40.0 |
| R0011 | 42.0 |
Pseudomonas Putida GPO1
Pseudomonas Putida GPO1 is growing 1 day on different substrates. We measured absorbance of the Pseudomonas Putida to see on which conditions the strain survives. Absorbance measurements
