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Team:Calgary/15 July 2010
From 2010.igem.org
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*Today I reconstructed K239000 with I13507 and I13504, then K135000 with I13507 and I13504 with proper plating. I also did a restriction enzyme digest of pRFP with EcoRI and HindIII and also XbaI and HindIII. And a gel was run of the restriction enzyme products. Also 4 tubes were prepared for I0500+B0034+E0040 to test inducing arabinose. | *Today I reconstructed K239000 with I13507 and I13504, then K135000 with I13507 and I13504 with proper plating. I also did a restriction enzyme digest of pRFP with EcoRI and HindIII and also XbaI and HindIII. And a gel was run of the restriction enzyme products. Also 4 tubes were prepared for I0500+B0034+E0040 to test inducing arabinose. | ||
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| + | <u>Alex</u> | ||
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| + | Ran all three gels of the PCR reactions. The reaction containing the highest magnesium ion concentration yielded PCR in all 12 reactions. The DNA found was of the right size (~200)bp. | ||
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Revision as of 17:16, 16 July 2010
Thurday July 15, 2010
Raida
- Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.
PCR conditions: Stage 1: 94 degrees Celcius Stage 2; Step 1: 94 degrees 1 minute Stage 2; Step 2: 55 degrees 45 seconds Stage 2; Step 3: 72 degrees 1 minute 15 seconds Stage 3: 72 degrees Holding Temperature: 4 degrees Celcius
- I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.
Jeremy
- Today I reconstructed K239000 with I13507 and I13504, then K135000 with I13507 and I13504 with proper plating. I also did a restriction enzyme digest of pRFP with EcoRI and HindIII and also XbaI and HindIII. And a gel was run of the restriction enzyme products. Also 4 tubes were prepared for I0500+B0034+E0040 to test inducing arabinose.
Alex
Ran all three gels of the PCR reactions. The reaction containing the highest magnesium ion concentration yielded PCR in all 12 reactions. The DNA found was of the right size (~200)bp.
No notebook page exists for this date. Sorry!
