Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Genomic DNA purification)
(Group: Retinal)
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<br>Notes: DNA material from tube 27 was taken from the freezer. Primers were standard vr-vf2, also from freezer. PFU buffer with MgSO4 was used. No further MgSO4 was added. A TAQ control was done in a parallel experiment.
<br>Notes: DNA material from tube 27 was taken from the freezer. Primers were standard vr-vf2, also from freezer. PFU buffer with MgSO4 was used. No further MgSO4 was added. A TAQ control was done in a parallel experiment.
<br><br>Protocol was followed to point, with the exeption of 1ul additional H2O being added because of an error. It will yield 51ul.<br><br> Program was run according to protocol. elongation time was set to 1 minute and 30 seconds.<br><br>
<br><br>Protocol was followed to point, with the exeption of 1ul additional H2O being added because of an error. It will yield 51ul.<br><br> Program was run according to protocol. elongation time was set to 1 minute and 30 seconds.<br><br>
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A control on the same DNA was run using TAQ polymerase. It was run on the same protocol and program as a parallel experiment in the flagella group: [https://2010.igem.org/Team:SDU-Denmark/labnotes#Amplification_of_pSB3K3_with_Taq Amplification of pSB3K3 with taq]
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A control on the same DNA was run using TAQ polymerase. It was run on the same protocol and program as a parallel experiment in the flagella group: [https://2010.igem.org/Team:SDU-Denmark/labnotes#Amplification_of_pSB3K3_with_Taq Amplification of pSB3K3 with taq]<br><br>
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=== Miniprep of BBa_K274210 in pSB1A2 ===
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Start date: 14/07    End date: <br>
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''Methods:''  Fermentas GeneJET plasmid miniprep kit <br><br>
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''Protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.1 MP1.1] <br><br>
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''Experiment done by:'' LC <br><br>
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''Notes:'' No pellet after step 4, but still continued. Later we got a small pellet at step 6. <br><br>
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''Results:''<br>
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The miniprep failed as expected, since there was no pellet after the second centrifugation.(insert gel picture)<br><br>
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''Analysis:''The experiment will be repeated in the afternoon.<br>
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Second try:
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Methods and protocol as in the first try.<br>
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''Notes:''

Revision as of 09:57, 14 July 2010