Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Genomic DNA purification)
(Extraction of BBa_B0034 from pSB1A2 plasmid using PFU)
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Experiment done by: Christian<br>
Experiment done by: Christian<br>
<br>Notes: DNA material from tube 27 was taken from the freezer. Primers were standard vr-vf2, also from freezer. PFU buffer with MgSO4 was used. No further MgSO4 was added. A TAQ control was done in a parallel experiment.
<br>Notes: DNA material from tube 27 was taken from the freezer. Primers were standard vr-vf2, also from freezer. PFU buffer with MgSO4 was used. No further MgSO4 was added. A TAQ control was done in a parallel experiment.
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<br><br>Protocol was followed to point, with the exeption of 1ul additional H2O being added because of an error. It will yield 51ul.<br><br> Program was run according to protocol. elongation time was set to 1 minute and 30 seconds.
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<br><br>Protocol was followed to point, with the exeption of 1ul additional H2O being added because of an error. It will yield 51ul.<br><br> Program was run according to protocol. elongation time was set to 1 minute and 30 seconds.<br><br>
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A control on the same DNA was run using TAQ polymerase. It was run on the same protocol and program as a parallel experiment in the flagella group: [https://2010.igem.org/Team:SDU-Denmark/labnotes#Amplification_of_pSB3K3_with_Taq Amplification of pSB3K3 with taq]

Revision as of 09:37, 14 July 2010