Team:Heidelberg/Notebook/ViroBytes/October
From 2010.igem.org
(→27/09/2010) |
(→27/09/2010) |
||
Line 143: | Line 143: | ||
Touchdown Phusion HiFi PCR was performed according to the following protocol: | Touchdown Phusion HiFi PCR was performed according to the following protocol: | ||
+ | |||
................................................ | ................................................ | ||
Line 169: | Line 170: | ||
................................................ | ................................................ | ||
+ | |||
+ | [[Image:Full_assembly_27.10.10.jpg|thumb|600px|right|]] | ||
**Gel loading scheme: | **Gel loading scheme: | ||
Line 179: | Line 182: | ||
**C 1-8 - fragments 1-8 control | **C 1-8 - fragments 1-8 control | ||
- | + | ||
{{:Team:Heidelberg/Single_Bottom}} | {{:Team:Heidelberg/Single_Bottom}} | ||
{{:Team:Heidelberg/Single_Bottom}} | {{:Team:Heidelberg/Single_Bottom}} |
Revision as of 03:22, 28 October 2010
October4/10/2010
5/10/2010
25/10/2010
PCR was set up as follows: 10 ul Phusion HF Buffer 5x 1ul of dNTP 0.5 ul of 100 um primers 2 ul of AAV template 36 ul of water ................................................ 98 °C/45 s ................................................ (1x 98 °C/15 s 72 °C/30 s ................................................ (35x) 72 °C/10 min ................................................ 4 °C/ hold
26/10/2010
Touchdown Phusion HiFi PCR was performed according to the following protocol: ................................................ 98 °C/30s ................................................ (1x 98 °C/15 s 72 °C/15 s (- 1.0 °C/cycle) 72 °C/1 min ................................................ (17x) 98 °C/15 s 55 °C/30 s 72 °C/1 min ................................................ (20x) 72 °C/10 min ................................................ (1x) 4 °C/ hold ................................................ 27/09/2010AAV 1 fragments 1-4; 5-8 and 1-8 (full capsid) assembly Touchdown Phusion HiFi PCR was performed according to the following protocol: ................................................ 98 °C/30s ................................................ (1x 98 °C/15 s 72 °C/15 s (- 1.0 °C/cycle) 72 °C/1 min ................................................ (17x) 98 °C/15 s 55 °C/30 s 72 °C/1 min ................................................ (20x) 72 °C/10 min ................................................ (1x) 4 °C/ hold ................................................
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||