"
Team:Newcastle/7 July 2010
From 2010.igem.org
(Difference between revisions)
RachelBoyd (Talk | contribs) (→Conclusion) |
PhilipHall (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| + | {{Team:Newcastle/mainbanner}} | ||
| + | |||
===Results=== | ===Results=== | ||
We used Gel Electrophoresis to test whether we have the DNA we wanted. As we already know, the ''ara'' gene is about 300bps. We used hundred bp DNA ladder as a guidance for our bands. The two bands produced were from two separate ''ara'' cultures and they indeed show the bands in the same region, i.e. 300bps. | We used Gel Electrophoresis to test whether we have the DNA we wanted. As we already know, the ''ara'' gene is about 300bps. We used hundred bp DNA ladder as a guidance for our bands. The two bands produced were from two separate ''ara'' cultures and they indeed show the bands in the same region, i.e. 300bps. | ||
| Line 6: | Line 8: | ||
===Conclusion=== | ===Conclusion=== | ||
The aim of this whole experiment was to extract genomic DNA from ''B. subtilis'' strain ATCC 6633. In order to test whether we had extracted the DNA, we first used PCR to amplify and then used Gel Electrophoresis to compare the bands of ''ara'' genes to the hundred bps ladder.It worked! | The aim of this whole experiment was to extract genomic DNA from ''B. subtilis'' strain ATCC 6633. In order to test whether we had extracted the DNA, we first used PCR to amplify and then used Gel Electrophoresis to compare the bands of ''ara'' genes to the hundred bps ladder.It worked! | ||
| + | {{Team:Newcastle/footer}} | ||
Revision as of 11:08, 2 August 2010
| |||||||||||||
| |||||||||||||
Results
We used Gel Electrophoresis to test whether we have the DNA we wanted. As we already know, the ara gene is about 300bps. We used hundred bp DNA ladder as a guidance for our bands. The two bands produced were from two separate ara cultures and they indeed show the bands in the same region, i.e. 300bps.
Conclusion
The aim of this whole experiment was to extract genomic DNA from B. subtilis strain ATCC 6633. In order to test whether we had extracted the DNA, we first used PCR to amplify and then used Gel Electrophoresis to compare the bands of ara genes to the hundred bps ladder.It worked!
   
|
    
|
