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Team:TU Delft/13 July 2010 content
From 2010.igem.org
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By Pieter | By Pieter | ||
| - | Today the digestion products were run on gel to see whether the plasmids were cut in the right way. | + | Today the digestion products from [[Team:TU_Delft/12_July_2010|yesterday]] were run on gel to see whether the plasmids were cut in the right way. |
[[Image:TU_Delft_2010-07-13_P_Digestion.png|thumb|left|600px|1% Agarose gel runned at 100V for 1h]] | [[Image:TU_Delft_2010-07-13_P_Digestion.png|thumb|left|600px|1% Agarose gel runned at 100V for 1h]] | ||
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The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel. | The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel. | ||
| + | |||
| + | ====Transformation=== | ||
| + | The ligation products that were incubated over night were transformed to TOP10 competent cells according to the protocol (@TODO: link!). | ||
Revision as of 14:25, 13 July 2010
Emulsifier
By Pieter
Today the digestion products from yesterday were run on gel to see whether the plasmids were cut in the right way.
Lane description
| # | Description | Length (bp) |
| 1 | Biorad EZ marker | |
| 2 | Undigested plasmid backbone | |
| 3 | Digested plasmid backbone [http://partsregistry.org/Part:pSB1T3 pSB1T3] | 2500 |
| 4 | Undigested AlnA | |
| 5 | Digested AlnA | 1107 |
| 6 | Undigested OprG | |
| 7 | Digested OprG | 744 |
| 8 | Undigested B0015 | |
| 9 | Digested [http://partsregistry.org/Part:BBa_B0015 B0015] | 130 |
| 10 | Undigested R0011 ! | |
| 11 | Digested [http://partsregistry.org/Part:BBa_R0011 R0011] | 55 |
| 12 | Undigested B0032 | |
| 13 | Digested [http://partsregistry.org/Part:BBa_B0032 B0032] | 13 |
| 14 | SmartLadder |
! Sample not fully loaded on gel
The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.
=Transformation
The ligation products that were incubated over night were transformed to TOP10 competent cells according to the protocol (@TODO: link!).
