Team:Imperial College London/Modelling/Output/Objectives

From 2010.igem.org

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#Which enzymes should be used as 3rd amplifying step? TEV (common enzyme for both 2 and 3 amplification step) or HIV1 (all three enzymes at three amplification levels are different)?
#Which enzymes should be used as 3rd amplifying step? TEV (common enzyme for both 2 and 3 amplification step) or HIV1 (all three enzymes at three amplification levels are different)?
In order to answer these questions, we would have to model this system. The results of the computer models would enable us to decide which design would be the most efficient one. This design would then be put forward to be built in labs.
In order to answer these questions, we would have to model this system. The results of the computer models would enable us to decide which design would be the most efficient one. This design would then be put forward to be built in labs.
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|The blue shadow indicates which part of the whole system that the fast response model simulates.
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|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;" align="right"|[[Team:Imperial_College_London/Modelling/Output/Detailed_Description | Click here for a detailed description of this model...]]
|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;" align="right"|[[Team:Imperial_College_London/Modelling/Output/Detailed_Description | Click here for a detailed description of this model...]]
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Revision as of 00:55, 28 October 2010

Modelling Overview | Detection Model | Signaling Model | Fast Response Model | Interactions
A major part of the project consisted of modelling each module. This enabled us to decide which ideas we should implement. Look at the Fast Response page for a great example of how modelling has made a major impact on our design!
Objectives | Description | Results | Constants | MATLAB Code
Objectives
Our novel concept of amplifying our output by incorporating enzymes raised some important questions that needed to be answered:
  1. How beneficial is it to incorporate the enzyme amplification step? We need to compare the speed of response between transcription and translation with 1-, 2- or 3-step amplification.
  2. How many amplification steps are beneficial? Will the addition of further amplification steps introduce considerable time delays?
  3. For how many amplification steps addition of another one does not reduce the speed of response very much?
  4. Which enzymes should be used as 3rd amplifying step? TEV (common enzyme for both 2 and 3 amplification step) or HIV1 (all three enzymes at three amplification levels are different)?

In order to answer these questions, we would have to model this system. The results of the computer models would enable us to decide which design would be the most efficient one. This design would then be put forward to be built in labs.

IC AIP threshold.png
The blue shadow indicates which part of the whole system that the fast response model simulates.
Click here for a detailed description of this model...
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