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Team:Newcastle/Scanning EM
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Swoodhouse (Talk | contribs) (New page: {{Team:Newcastle/mainbanner}} =Scanning EM== This is a standard method for Scanning Electro Microscopy. Specimens vary greatly and the final protocol should be decided by referring to te...) |
Swoodhouse (Talk | contribs) (→Scanning EM=) |
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=Scanning EM== | =Scanning EM== | ||
| - | This is a standard method for Scanning | + | This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications. |
==Fixation== | ==Fixation== | ||
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100% ethanol: 1 hr. | 100% ethanol: 1 hr. | ||
| - | Once in | + | Once in 100% ethanol, samples are dried using a Baltec Critical Point Dryer. |
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| + | ==Mounting== | ||
| + | Mount of an aluminium stub with Achesons Silver ElectroDag. | ||
| + | |||
| + | ==Gold coating== | ||
| + | Coat with 15nm of gold using a Polaron SEM Coating Unit. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 22:01, 27 October 2010
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Contents |
Scanning EM=
This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications.
Fixation
Fix in 2% Gluteraldehyde in Sorensons Phosphate Buffer overnight (minimum). Rinse in Sorensons Phosphate Buffer 2x 15 mins.
Dehydration
25% ethanol: 30 mins. 50% ethanol: 30 mins. 75% ethanol: 30 mins. 100% ethanol: 1 hr. 100% ethanol: 1 hr.
Once in 100% ethanol, samples are dried using a Baltec Critical Point Dryer.
Mounting
Mount of an aluminium stub with Achesons Silver ElectroDag.
Gold coating
Coat with 15nm of gold using a Polaron SEM Coating Unit.
   
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