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Team:Newcastle/23 June 2010
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Revision as of 14:17, 27 July 2010
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Contents |
Wednesday Morning
Aims
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.
Equipment List
- E.coli broth culture
- Pipettes
- Plasmid ligation products from yesterday
- Conical flasks
Transformation
Cells are made competent ready for transformation.
- Positive control(plasmid uncut)- to make sure cells are transfroming properly
- Negative control (just cells)- nothing but competent cells
5 microfuge tubes:
- 1:5
- 1:3
- Vector
- Negative add nothing
- Positive add 1μl uncut
Wednesday afternoon taking a break
Aims
The aim of this Lab practice was to have fun.
Equipment list
- Candy floss
- Suntan lotion
- Teddy bears
- Burgers and bacon
- Chips and gravy
- Icecream
- Meerkat toys
- Madmouse ride!
- Beer, wine, lemonade
- Football
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