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Team:Chiba/lux promoter
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==Abstract== | ==Abstract== | ||
| - | + | LuxRはAHL依存的な転写活性を持つ。 | |
| - | LuxR is an AHL- | + | LuxR is an AHL-dependent activator. However insert lux box between -35 and -10 and LuxR fuctions as LuxR-AHL inverter. |
| - | Someone resistered the Plux inverter in Biobrick | + | Someone resistered the Plux inverter in Biobrick.We've prepare Plux inv-GFP and characterized about it.<br> |
==Experiments== | ==Experiments== | ||
Revision as of 20:57, 27 October 2010
Contents |
Abstract
LuxRはAHL依存的な転写活性を持つ。
LuxR is an AHL-dependent activator. However insert lux box between -35 and -10 and LuxR fuctions as LuxR-AHL inverter.
Someone resistered the Plux inverter in Biobrick.We've prepare Plux inv-GFP and characterized about it.
Experiments
1.Preparing Plux inv-GFP
1．Plux inv－GFPの作製 クローニングのprocessをFig. 1に示す。作製したPlux inv-GFPを形質転換した株は緑色蛍光を示した。また，sequenceも正しいことを確認した。 Preparing Plux inv-GFP process is shown in Fig. 1.
Fig. 1 Evaluation of LuxR Inverter
2.Characterization Plux inv-GFP
Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。コロニーをつついてLB液体培地(AHL0および1000 nM)で12 h培養し, スピンダウンしてペレットを観察した。
Fig. 2 assay protocol
Results
Reference
- Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
- Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)
