"
Team:Newcastle/Meetings/7 July 2010
From 2010.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
==Research feedback== | ==Research feedback== | ||
* Swarming function. ''sfP'' and ''swrA'' genes needed from 3610 marburg strain. Swarming depends on the two-component system of ''comP'' and ''comA'' which is activated by ''comX'' and ''csF''. ComA then activate srfABCD, leadint to surfactin production. ''comS'' will also be activated which is for competence. Surfactin may break the surface tension to allow movement. Frame shift mutation is the ''sfP'' gene which is needed for the post translational modification of the surfactin. Mutation in the ''swrA'' too, which is needed for the flagella biosynthesis. Surfactin also an amp, however the immunity gene is found in the 168 strain. | * Swarming function. ''sfP'' and ''swrA'' genes needed from 3610 marburg strain. Swarming depends on the two-component system of ''comP'' and ''comA'' which is activated by ''comX'' and ''csF''. ComA then activate srfABCD, leadint to surfactin production. ''comS'' will also be activated which is for competence. Surfactin may break the surface tension to allow movement. Frame shift mutation is the ''sfP'' gene which is needed for the post translational modification of the surfactin. Mutation in the ''swrA'' too, which is needed for the flagella biosynthesis. Surfactin also an amp, however the immunity gene is found in the 168 strain. | ||
| + | * Gibson cloning to disrupt the frame shift mutation. Neil, Jem and Wendy is working on the Gibson method. Faster way to sticking the biobrick together. | ||
==Lab feedback== | ==Lab feedback== | ||
| Line 10: | Line 11: | ||
* Urea tests - Harsh and Alan. Pink colour change observed. Urea being breakdown by ''B subtilis''. | * Urea tests - Harsh and Alan. Pink colour change observed. Urea being breakdown by ''B subtilis''. | ||
* DNA extraction - Da and Deena. Extract DNA from strain 3633. Needed for ''rocF'' and ''spa'' genes. | * DNA extraction - Da and Deena. Extract DNA from strain 3633. Needed for ''rocF'' and ''spa'' genes. | ||
| - | * LacI extraction - | + | * LacI extraction - Phil and Younus. Extracted ''lacI'' from pMutin4 plasmid. |
| + | |||
| + | ==Logo and T-shirts== | ||
| + | * Put CBCB logo on the t-shirt | ||
| + | |||
| + | ==Visas== | ||
| + | * Harsh to travel to London on 160710. | ||
| + | |||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 12:45, 12 July 2010
| |||||||||||||
| |||||||||||||
Contents |
Roll call
Everyone here except Racheal, Colin and Jennifer.
Research feedback
- Swarming function. sfP and swrA genes needed from 3610 marburg strain. Swarming depends on the two-component system of comP and comA which is activated by comX and csF. ComA then activate srfABCD, leadint to surfactin production. comS will also be activated which is for competence. Surfactin may break the surface tension to allow movement. Frame shift mutation is the sfP gene which is needed for the post translational modification of the surfactin. Mutation in the swrA too, which is needed for the flagella biosynthesis. Surfactin also an amp, however the immunity gene is found in the 168 strain.
- Gibson cloning to disrupt the frame shift mutation. Neil, Jem and Wendy is working on the Gibson method. Faster way to sticking the biobrick together.
Lab feedback
- Arginine tests - Harsh and Alan. No pH change observed. Use a minimal medium, as other nitrogen source might be present in LB.
- Urea tests - Harsh and Alan. Pink colour change observed. Urea being breakdown by B subtilis.
- DNA extraction - Da and Deena. Extract DNA from strain 3633. Needed for rocF and spa genes.
- LacI extraction - Phil and Younus. Extracted lacI from pMutin4 plasmid.
Logo and T-shirts
- Put CBCB logo on the t-shirt
Visas
- Harsh to travel to London on 160710.
   
|
    
|
