Lab notes (7/12 - 7/18)

Group: Flagella

Extraction of psb3k3 plasmids with incerted RFP from E. coli MG1655 (log phase)

Methods: MiniPrep, NanoDrop and gel electrophoresis

Notes: We used 9 ml ON culture, loaded 2ul sample and 8ul agarose loading dye on a 1,5% gel,used a DNA ladder mix (100-10000 nucleotides) as marker

Results: Nanodrop: Tube 1: 30.7 mg/ul Tube 2: 27.4 mg/ul

Gel electrophoresis: No result

--Louch07 15:00, 9 July 2010 (UTC)

Group: Photosensor

Group: Retinal

Colony PCR of K274210 in pSB1A2 (transformation from 08/07)

Methods: Colony PCR

Protocol: Modified iGEM colony PCR protocol

Notes: MgCl2 was added as a gradient,sample 1-2 contain 2 ul (0,04255), sample 3-5 2,25ul (0,04787). We mistakenly added 30ul (instead of 17ul) premix to sample 6 - 9, so the concentration of MgCl2 is reduced. Sample 6 contains 2,25ul (0,0375), Sample 7-8 2,5ul (0,04167), Sample 9 2,75ul (0,04583).

Results:

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