May 26

Make spike and HNMT plasmid and transform bacteria with it. Bacteria expressing it will produce HNMT and engulf via membrane fusion. Ultimately, use it as a spray. What we should do (June 1st~August 31th)

1. Syenthesis of bacteria
   • Company: S.B. Yang>contact, rest>look for companies. Contact T.A.
   • Sequence source: D.C. Yang, K.K. Kim
   • 1),2): by June 1st
   • KI building (Prof. Choi)
2. Homepage design
   • K.K. Kim, +alpha
   • SPARCS
   • I.D. department
   • 2),3): cost a lot - about \1million. stick with 1)
   • S.B. Yang, K.K. Kim, D.C. Yang, H.S. Kim handle program until June 6th. The rest (design team) design.
3. Daily update
4. Confirmation of the product
   • Florescent material such as GFP
   • Quantum dot

May 27

1st solid idea: respiratory system using spike protein & HNMT We have to make two plasmid which would be guide plasmid and functional plasmid. We put spike gene in guide plasmid and HNMT in functional plasmid. Then bacteria will get spikes on outer membrane and HNMT inside the cell. After that if we can pack HNMT in the vesicle. Then we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.

Do list

• See Stanford-secretion gene (everyone)
• Find potential genes

Literature: H.S. Kim, S.H. Kim, S.J. Kim, Triple Yangs

Company: rest

by Saturday

• KI workshop next Friday
• DNA kit will arrive. Homepage design team assigned.
• Potential name: FADEX (Fast Accurate Drug Exporting System)
• Everyday 8 o'clock