Team:Heidelberg/Project/Mouse Infection
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===procedure involving animals=== | ===procedure involving animals=== | ||
- | The mouse experiments were conducted in accordance with the animal facility of the [https://2010.igem.org/Team:Heidelberg/Team/Institutes german cancer research institute of Heidelberg]. Female NMRI mice were | + | The mouse experiments were conducted in accordance with the animal facility of the [https://2010.igem.org/Team:Heidelberg/Team/Institutes german cancer research institute of Heidelberg]. Female NMRI mice were obtained from a collaboration with Dr. Oliver Müller. At 8-10 weeks of age, the animals were injected in the tail vein (TV), with <nowiki>~</nowiki> 1x10<sup>11</sup> particles of AAV-SV40-luciferase in 200µl of 1x phosphate-buffered saline. The mice are transferred to a holding device which restrains the mouse while allowing access to the tail vein. The tails were warmed before the injections and injections were carried out using 27 gauge needles. All the mice recoverd from the injection quickly without loss of mobility or interruption of grooming activity {{HDref|Zincarelli et al., 2008}}. |
===in vivo animal imaging=== | ===in vivo animal imaging=== |
Revision as of 17:58, 27 October 2010
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