Team:Chiba/lux promoter

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  <td width="800px"><font size="5" face=verdana>Abstract</font><br>
 
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BBa_R0061は-35および-10の間にlux box を含む配列である。LuxR-AHL複合体によって下流の遺伝子転写を抑制する。 <br>
 
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R0061 contains lux box -35 and -10 is LuxR-AHL inverter. <br>
 
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There is promoter (HSL-luxR repressor), biobrick number R0061.<br>
 
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We've prepare Plux inv-GFP and characterized about it.<br><br>
 
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==Abstract==
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BBa_R0061は-35および-10の間にlux box を含む配列である。LuxR-AHL複合体によって下流の遺伝子転写を抑制する。<br>
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R0061 contains lux box -35 and -10 is LuxR-AHL inverter.
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There is promoter (HSL-luxR repressor), biobrick number R0061.
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We've prepare Plux inv-GFP and characterized about it.
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==Experiments==
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===1.Preparing Plux inv-GFP===
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  <td width="800px"><font size="5" face=verdana>Experiments</font><br>
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<font size="3">1.Preparing Plux inv-GFP</font><br>
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&nbsp;&nbsp;
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Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed.
Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed.
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[[Image:Plux inv-GFP.png|frame|center|Fig. 1 cloning process]]
[[Image:Plux inv-GFP.png|frame|center|Fig. 1 cloning process]]
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<font size="3">2.Characterization Plux inv-GFP</font>
 
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===2.Characterization Plux inv-GFP===
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[[Image:lux inverter function.png|frame|center|Fig. 2 assay process]]
[[Image:lux inverter function.png|frame|center|Fig. 2 assay process]]
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&nbsp;&nbsp; Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。
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Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。  
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==Results==
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[[Image:Plux inv results.png|200px|]]
[[Image:Plux inv results.png|200px|]]
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==Reference==
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#Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
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#Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)
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<br>1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
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<br>2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)
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Revision as of 15:08, 27 October 2010




Contents

Abstract

BBa_R0061は-35および-10の間にlux box を含む配列である。LuxR-AHL複合体によって下流の遺伝子転写を抑制する。
R0061 contains lux box -35 and -10 is LuxR-AHL inverter. There is promoter (HSL-luxR repressor), biobrick number R0061. We've prepare Plux inv-GFP and characterized about it.

Experiments

1.Preparing Plux inv-GFP

Preparing Plux inv-GFP process is shown in Fig. 1.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed.

Fig. 1 cloning process


2.Characterization Plux inv-GFP

Fig. 2 assay process

Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。

Results

Plux inv results.png

Reference

  1. Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
  2. Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)
Retrieved from "http://2010.igem.org/Team:Chiba/lux_promoter"