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Team:Calgary/9 July 2010
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| - | Today I did a restriction digest of parts with Eco/Spe in buffer | + | Today I did a restriction digest of parts with Eco/Spe in buffer II that I constructed and the plasmid switch I did on July 6th. |
The parts that I digested were: | The parts that I digested were: | ||
*3 colonies of E1010 in AC plasmid | *3 colonies of E1010 in AC plasmid | ||
*2 colonies of E1010-B0015 construction done on July 6th | *2 colonies of E1010-B0015 construction done on July 6th | ||
*I also ran 2 colonies of I13507 | *I also ran 2 colonies of I13507 | ||
| - | *1 colony of E1010 | + | *1 colony of E1010 in its original Kanamycin plasmid |
Refer to the image for more details about the restriction digest. | Refer to the image for more details about the restriction digest. | ||
| Line 18: | Line 18: | ||
I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week. | I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week. | ||
| - | I also designed primer along with Dave for the | + | I also designed primer along with Dave for the ppRFP circuit. |
}} | }} | ||
Revision as of 20:11, 9 July 2010
restriction digest of parts that Himika ran on 07-09-2010
Friday July 9, 2010
Himika
Today I did a restriction digest of parts with Eco/Spe in buffer II that I constructed and the plasmid switch I did on July 6th. The parts that I digested were:
- 3 colonies of E1010 in AC plasmid
- 2 colonies of E1010-B0015 construction done on July 6th
- I also ran 2 colonies of I13507
- 1 colony of E1010 in its original Kanamycin plasmid
Refer to the image for more details about the restriction digest.
I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week.
I also designed primer along with Dave for the ppRFP circuit.
No notebook page exists for this date. Sorry!
