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Team:Chiba/lux promoter
From 2010.igem.org
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| - | + | BBa_R0061は-35および-10の間にlux box を含む配列である。LuxR-AHL複合体によって下流の遺伝子転写を抑制する。 <br> | |
| + | R0061 contains lux box -35 and -10 is LuxR-AHL inverter. <br> | ||
There is promoter (HSL-luxR repressor), biobrick number R0061.<br> | There is promoter (HSL-luxR repressor), biobrick number R0061.<br> | ||
We've prepare Plux inv-GFP and characterized about it.<br><br> | We've prepare Plux inv-GFP and characterized about it.<br><br> | ||
Revision as of 14:50, 27 October 2010
| Abstract BBa_R0061は-35および-10の間にlux box を含む配列である。LuxR-AHL複合体によって下流の遺伝子転写を抑制する。 R0061 contains lux box -35 and -10 is LuxR-AHL inverter. There is promoter (HSL-luxR repressor), biobrick number R0061. We've prepare Plux inv-GFP and characterized about it. |
| Experiments 1.Preparing Plux inv-GFP Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed. |
Fig. 1 cloning process
| 2.Characterization Plux inv-GFP |
Fig. 2 assay process
Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。
Results
Reference |
1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000) 2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007) |
