Team:Warsaw/Stage1

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<h2>RbSs' measurement</h2>
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<h2>Gene expression control</h2>
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<div class="note">All substances are poisons. There is none which is not a poison. The right dose differentiates a poison and a remedy. Paracelsus</div>
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<p>In order to make our device - the BactoDHL functioning correctly we had to express each gene in the right dose. To high expression of certain proteins, especially the LLO would be toxic to the cells. To low expresion would result in poor performance of our device. </p>
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<p>Because our devices are encoded as operons we have in fact faced two different problems at two different levels:</p>
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<img src="https://static.igem.org/mediawiki/2010/d/db/Operon_inv.png"/>
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<ul>
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<li>Global expression regulation of the whole operon which is controlled at promoter level</li>
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<li>Local expression regulation of each gene in the operon, which is controlled at the RBS level</li>
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</ul>
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Revision as of 19:58, 16 October 2010

Example Tabs

Gene expression control

All substances are poisons. There is none which is not a poison. The right dose differentiates a poison and a remedy. Paracelsus

In order to make our device - the BactoDHL functioning correctly we had to express each gene in the right dose. To high expression of certain proteins, especially the LLO would be toxic to the cells. To low expresion would result in poor performance of our device.

Because our devices are encoded as operons we have in fact faced two different problems at two different levels:

  • Global expression regulation of the whole operon which is controlled at promoter level
  • Local expression regulation of each gene in the operon, which is controlled at the RBS level

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