Team:SDU-Denmark/project-i

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(Hyperflagellation)
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== Hyperflagellation ==
== Hyperflagellation ==
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To achieve hyperflagellation we have decided to focus mainly on increasing the expression of the ''flhD'' and ''flhC'' transcriptional regulators, also known as the master regulon of flagella synthesis [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 7]]. In normal ''E. coli'' the ''flhDC'' operon is tightly regulated by numerous factors [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 7]], resulting in average expression of 4 flagella per cell [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 8]]. In some hyperflagellated strains, mutations have been found upstream of the regulon that increase expression[[https://2010.igem.org/Team:SDU-Denmark/project-i#References 9]], making the cells hypermotile. We have decided to take a down-and-dirty approach to increase flagella expression, overriding the regulation alltogether by putting the two genes on a constitutive promotor. We hereby hope to increase the pumping power of our system.
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To achieve hyperflagellation we have decided to focus mainly on increasing the expression of the ''flhD'' and ''flhC'' transcriptional regulators, also known as the master regulon of flagella synthesis [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 7]]. In normal ''E. coli'' the ''flhDC'' operon is tightly regulated by numerous factors [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 7]], resulting in average expression of 4 flagella per cell [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 8]]. In some hyperflagellated strains, mutations have been found upstream of the regulon that increase expression [[https://2010.igem.org/Team:SDU-Denmark/project-i#References 9]], making the cells hypermotile. We have decided to take a down-and-dirty approach to increase flagella expression, overriding the regulation alltogether by putting the two genes on a constitutive promotor. We hereby hope to increase the pumping power of our system.
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Revision as of 11:48, 27 October 2010