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Team:Newcastle/Meetings/1 April 2010
From 2010.igem.org
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Contents |
Formal Meeting - 1st April 2010
- Room: CBCB; Start: 4 p.m.
- Chair: Da, Minutes: Alan, Computer: Younus
- Agenda
Roll call and apologies
- Present: Jen, Janetta, Steven, Younus, Da, Alan, Richard, Rachel, Jem, Matt
- Apologies: Phil, Harsh and Zoltan (Holiday); Neil
Approval of agenda and minutes
- Approved
Research Feedback
Nutrient
- Using a glucose and starch concentration gradient. Uses the catabolic repression system to allow glue production. *Have to test and find suitable starch and chemoattractant!!!!!!!!
Sporulation, germination and secretion control
- AbrB gene maybe a suitable gene to repress sporulation by overexpressing it.
- Can also activate antimicrobial peptides like TasA.
- Need to find what other genes that are involved in the pathway of AbrB.
Quorum Sensing
- Number of competence cells not an issue. Sin operone to control a lot of pathway.
Other points
- FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis.
- Is spider silk able to resist the alkali conditions?
- Is it possible to produce spider silk in Bacillus?
Voigt paper feedback
- Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes.
- Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system.
Travel Plans
- Too early to book flights and hotels. May have other members joining.
- Visa/Passport update: Harsh to apply on May, June period
Tutorial
Next tutorial to be on the week after
Flowchart
- Flow Chart
- Need to be more in depth. i.e. The problems, what the bacteria need to do to achieve the goal and the genes that are needed and why.
- Another flow chart to show the different stituation and how the bacteria response to it.
Other business
- Foxit not working
- Sponsors on T-shirt: contact sponsors next week (Jannetta)
Action points
- To draft email to Colin and Chris and show it to instructors first(everyone)
- To continue to develop flow chart(everyone)
- Tutorial on Wiki
Next Meeting
- Informal meeting: 070410 at 11 a.m. in Walton library
- Formal meeting: 070410 at 3 p.m. in CBCB
- Chair:Richard, Minutes: Rachel, Computer:Jannetta
