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Team:Chiba/lux promoter
From 2010.igem.org
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Revision as of 08:03, 27 October 2010
Lux promoter inverter
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We've charaterized Plux inverter.
There is promoter (HSL-luxR repressor), biobrick number R0061.
We've made Plux inv-GFP and characterized about it.
Experiments
1,Preparing Plux inx-GFP
cloning process is shown in Fig. 1
Fig. 1 cloning process
Results
Fig. 2 assay process
1) Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)
