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Team:Queens-Canada/16 August 2010
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(New page: <h2>August 16, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Yuli '''pMec-7'''::'''eGFP''' Program run using 9:1 FAST:HIFI blend with an exten...) |
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Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | Practiced injections with Sur-5::GFP marker. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 02:03, 28 October 2010
August 16, 2010
PCR
Yuli
pMec-7::eGFP
Program run using 9:1 FAST:HIFI blend with an extension time of 20 s and annealing temperature of 60 °C.
Miniprep
Thai and Chris Culture tubes from 13/08/10
Gel Electrophoresis
Thai
Used 0.3 g agarose in 40 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Yuli
pSip-1, pRab-7, mCherry, pFlp-1
Gel Electrophoresis
Thai and Yuli
pCeh-12::1C3, ChR2::eYFP::1C3, eGFP, pSB1C3, ChR2::eYFP::unc-54 3'UTR::1T3
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
