Team:Queens-Canada/13 August 2010

Liquid Culture

Hao and Thai and Yuli and Chris

pSip-1::1C3, pRab-7::1C3, mCherry::1C3, pFlp-1::1C3, unc-54 3'UTR::1A3, pOdr-10::1C3, ChR2::1C3, pCeh-12::1C3, ChR2::eYFP::unc-54 3'UTR::1T3, ChR2::eYFP::1C3, eGFP::1C3

All plates had ~1000:1 red:white colony ratio. Most plates had less than 10 white colonies.

Digestion-Ligation

Hao and Thai

pMec-7, pSra-10, pSrb-6, pHsp-3, eYFP, pStr-220, pSB1C3

Cut with EcoRI and PstI. Backbone treated with CIAP for 30 mins. T4 ligase used.

PCR

Yuli

NpHR::eCFP

Program run using 9:1 FAST:HIFI blend with an extension time of 25 s and annealing temperature of 58 °C.

PCR

Yuli

pSip-1::eGFP, pRab-7::eGFP