Team:Queens-Canada/17 June 2010

From 2010.igem.org

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Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.
Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.
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2010
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<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3>
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Digestion
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Nelson
Nelson

Revision as of 05:23, 27 October 2010

Contents

June 17, 2010

Gel Electrophoresis

Kevin and Nelson

pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP

Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.

Digestion

Nelson

BBa_J45200, BBa_J45120

Incubated at 37 °C for 20 mins. Products stored at -20 °C.

Gel Electrophoresis

Yuli PCR Purification products from June 16, 2010

Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V.