Team:Caltech/Parts

From 2010.igem.org

(Difference between revisions)
(Added Intermediate Parts section)
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== Intermediate Parts ==
== Intermediate Parts ==
<!-- Following code generated by CompletedPartsFormatter, (c) 2010 Alexandre Boulgakov -->
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{| class="wikitable" style="margin: 1em auto 1em auto;"
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{{{!}} class="wikitable" style="margin: 1em auto 1em auto;"
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! scope="col" | Part Name
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! scope="col" {{!}} Part Name
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! scope="col" | Brick Number
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! scope="col" {{!}} Brick Number
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! scope="col" | Resistance
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! scope="col" {{!}} Resistance
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! scope="col" | Subparts
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! scope="col" {{!}} Subparts
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|-
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{{!}}-
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| phaB1.RBS (plastic intermediate) || <partinfo>BBa_K332023</partinfo> || Cm || <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>
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{{!}} phaB1.RBS (plastic intermediate) {{!}}{{!}} <partinfo>BBa_K332023</partinfo> {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| Heat-Shock Activated Promoter || <partinfo>BBa_K338001</partinfo> || Amp || Original part
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{{!}} Heat-Shock Activated Promoter {{!}}{{!}} <partinfo>BBa_K338001</partinfo> {{!}}{{!}} Amp {{!}}{{!}} Original part
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|-
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{{!}}-
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| Heat-Shock or IPTG Induced Generator || <partinfo>BBa_K338002</partinfo> || Kan || <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>
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{{!}} Heat-Shock or IPTG Induced Generator {{!}}{{!}} <partinfo>BBa_K338002</partinfo> {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| pLacI.RBS.phaA.RBS (plastic intermediate) || <partinfo>BBa_K338003</partinfo> || Kan || <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
+
{{!}} pLacI.RBS.phaA.RBS (plastic intermediate) {{!}}{{!}} <partinfo>BBa_K338003</partinfo> {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| phaB1.RBS.phaC1.TT (plastic intermediate) || <partinfo>BBa_K338004</partinfo> || Kan || <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} phaB1.RBS.phaC1.TT (plastic intermediate) {{!}}{{!}} <partinfo>BBa_K338004</partinfo> {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| phaA.RBS (plastic intermediate) || <partinfo>BBa_K338022</partinfo> || Cm || <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
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{{!}} phaA.RBS (plastic intermediate) {{!}}{{!}} <partinfo>BBa_K338022</partinfo> {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| phaC1 with Double Terminator || <partinfo>BBa_K338024</partinfo> || Cm || <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
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{{!}} phaC1 with Double Terminator {{!}}{{!}} <partinfo>BBa_K338024</partinfo> {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| +OmpR, +(CinR-HSL) Double Promoter || <partinfo>BBa_K338029</partinfo> || Tet || <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>
+
{{!}} +OmpR, +(CinR-HSL) Double Promoter {{!}}{{!}} <partinfo>BBa_K338029</partinfo> {{!}}{{!}} Tet {{!}}{{!}} <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>
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|-
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{{!}}-
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| GFP Reporter Device, Heat-Shock Induced || <partinfo>BBa_K338042</partinfo> || Kan || <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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{{!}} GFP Reporter Device, Heat-Shock Induced {{!}}{{!}} <partinfo>BBa_K338042</partinfo> {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| +Heat-Shock, +CinR-HSL Double Promoter || <partinfo>BBa_K338046</partinfo> || Kan || <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0077</partinfo>
+
{{!}} +Heat-Shock, +CinR-HSL Double Promoter {{!}}{{!}} <partinfo>BBa_K338046</partinfo> {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0077</partinfo>
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|-
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{{!}}-
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| GFP Reporter Device, Heat-Shock or IPTG Induced || <partinfo>BBa_K338062</partinfo> || Cm || <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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{{!}} GFP Reporter Device, Heat-Shock or IPTG Induced {{!}}{{!}} <partinfo>BBa_K338062</partinfo> {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| Heat-Shock Activated Generator || <partinfo>BBa_K338081</partinfo> || Kan || <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
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{{!}} Heat-Shock Activated Generator {{!}}{{!}} <partinfo>BBa_K338081</partinfo> {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| GFP Reporter Device, Cinr-HSL Induced || Not Submitted || Cm || <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} GFP Reporter Device, Cinr-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| HSL with Double Terminator || Not Submitted || Cm || <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
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{{!}} HSL with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| Constitutive Generator || Not Submitted || Kan || <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>
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{{!}} Constitutive Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| CinR with Double Terminator || Not Submitted || Cm || <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
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{{!}} CinR with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
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{{!}}-
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| +IPTG, +(CinR-HSL) Double Promoter || Not Submitted || Cm || <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
+
{{!}} +IPTG, +(CinR-HSL) Double Promoter {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
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|-
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{{!}}-
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| OmpR Induced Generator || Not Submitted || Tet || <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>
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{{!}} OmpR Induced Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Tet {{!}}{{!}} <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
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{{!}}-
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| HSL.TT.pLacI.p(CinR-HSL) (intermediate) || Not Submitted || Amp || <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
+
{{!}} HSL.TT.pLacI.p(CinR-HSL) (intermediate) {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
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|-
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{{!}}-
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| HSL Device, CinR-HSL Induced || Not Submitted || Kan || <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} HSL Device, CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| HSL Device || Not Submitted || Kan || <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} HSL Device {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| Heat-Shock Activated Generator || Not Submitted || Kan || <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
+
{{!}} Heat-Shock Activated Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
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|-
+
{{!}}-
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| GFP Reporter Device, OmpR Induced || Not Submitted || Cm || <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} GFP Reporter Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| GFP Reporter Device, Constitutive || Not Submitted || Kan || <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} GFP Reporter Device, Constitutive {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| K124014 Holin with Double Terminator || Not Submitted || Cm || <partinfo>BBa_K124014</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} K124014 Holin with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_K124014</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| K124017 Lysis Cassette with Double Terminator || Not Submitted || Cm || <partinfo>BBa_K124017</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} K124017 Lysis Cassette with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} <partinfo>BBa_K124017</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| CinR Device, OmpR or CinR-HSL Induced || Not Submitted || Amp || <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} CinR Device, OmpR or CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
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|-
+
{{!}}-
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| CinR Device, OmpR Induced || Not Submitted || Amp || <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
+
{{!}} CinR Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
-
|}
+
{{!}}}
<!-- End CompletedPartsFormatter section. -->
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Revision as of 02:13, 27 October 2010


iGEM 2010



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Completed Systems

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Completed Bricks

Heat shock promoter: BBa_K338001

HSP-LacI: BBa_K338002

Plastic, part 1: BBa_K338003

Plastic, part 2: BBa_K338004

Intermediate Parts

Part Name Brick Number Resistance Subparts
phaB1.RBS (plastic intermediate) <partinfo>BBa_K332023</partinfo> Cm <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>
Heat-Shock Activated Promoter <partinfo>BBa_K338001</partinfo> Amp Original part
Heat-Shock or IPTG Induced Generator <partinfo>BBa_K338002</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>
pLacI.RBS.phaA.RBS (plastic intermediate) <partinfo>BBa_K338003</partinfo> Kan <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
phaB1.RBS.phaC1.TT (plastic intermediate) <partinfo>BBa_K338004</partinfo> Kan <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
phaA.RBS (plastic intermediate) <partinfo>BBa_K338022</partinfo> Cm <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
phaC1 with Double Terminator <partinfo>BBa_K338024</partinfo> Cm <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
+OmpR, +(CinR-HSL) Double Promoter <partinfo>BBa_K338029</partinfo> Tet <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>
GFP Reporter Device, Heat-Shock Induced <partinfo>BBa_K338042</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
+Heat-Shock, +CinR-HSL Double Promoter <partinfo>BBa_K338046</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0077</partinfo>
GFP Reporter Device, Heat-Shock or IPTG Induced <partinfo>BBa_K338062</partinfo> Cm <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
Heat-Shock Activated Generator <partinfo>BBa_K338081</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
GFP Reporter Device, Cinr-HSL Induced Not Submitted Cm <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
HSL with Double Terminator Not Submitted Cm <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
Constitutive Generator Not Submitted Kan <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>
CinR with Double Terminator Not Submitted Cm <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
+IPTG, +(CinR-HSL) Double Promoter Not Submitted Cm <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
OmpR Induced Generator Not Submitted Tet <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>
HSL.TT.pLacI.p(CinR-HSL) (intermediate) Not Submitted Amp <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
HSL Device, CinR-HSL Induced Not Submitted Kan <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
HSL Device Not Submitted Kan <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
Heat-Shock Activated Generator Not Submitted Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
GFP Reporter Device, OmpR Induced Not Submitted Cm <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
GFP Reporter Device, Constitutive Not Submitted Kan <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
K124014 Holin with Double Terminator Not Submitted Cm <partinfo>BBa_K124014</partinfo>, <partinfo>BBa_B0015</partinfo>
K124017 Lysis Cassette with Double Terminator Not Submitted Cm <partinfo>BBa_K124017</partinfo>, <partinfo>BBa_B0015</partinfo>
CinR Device, OmpR or CinR-HSL Induced Not Submitted Amp <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
CinR Device, OmpR Induced Not Submitted Amp <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>


Apparatus

The red/infrared light apparatus

The red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]).

We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42°C.

Stage of the light apparatus; temperature at 42°C.
 
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