Team:Heidelberg/Notebook/ViroBytes/October
From 2010.igem.org
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*~200ng of fragment DNA per reaction | *~200ng of fragment DNA per reaction | ||
*ligation time ~10min | *ligation time ~10min | ||
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+ | ==27/09/2010== | ||
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+ | AAV 1 fragments 1-4; 5-8 and 1-8 (full capsid) assembly | ||
+ | |||
+ | [[Image:Full_assembly_27.10.10.jpg|thumb|600px|right|]] | ||
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+ | |||
+ | **Gel loading scheme: | ||
+ | **M - GeneRuler 1kb DNA ladder | ||
+ | **F 1-4 - fragments 1-4 assembly | ||
+ | **F 5-8 - fragments 5-8 assembly | ||
+ | **F 1-8 - fragments 1-8 assembly | ||
+ | **C 1-4 - fragments 1-4 control | ||
+ | **C 5-8 - fragments 5-8 control | ||
+ | **C 1-8 - fragments 1-8 control | ||
+ | |||
+ | {{:Team:Heidelberg/Single_Bottom}} | ||
{{:Team:Heidelberg/Single_Bottom}} | {{:Team:Heidelberg/Single_Bottom}} |
Revision as of 03:10, 28 October 2010
![](https://static.igem.org/mediawiki/2010/9/92/Bg_plasmid.png)
October4/10/2010
5/10/2010
27/09/2010AAV 1 fragments 1-4; 5-8 and 1-8 (full capsid) assembly
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