Team:Heidelberg/Notebook/miRNA Kit/September
From 2010.igem.org
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[[Image:20100926 digestion check.png | thumb | 350px | right | Gel 100926-1. Analytical gel with following lanes: 1) R33 E/N (from 23<sup>rd</sup> September) 2) R23_1 S/P 3) R23_6 4) R33C 5) R23_4 6) R23_8 7) 1kb Plus Ladder (Invitrogen) 8) R33C E/N 9) R23_4 S/P 9) R23C_8 S/P]] | [[Image:20100926 digestion check.png | thumb | 350px | right | Gel 100926-1. Analytical gel with following lanes: 1) R33 E/N (from 23<sup>rd</sup> September) 2) R23_1 S/P 3) R23_6 4) R33C 5) R23_4 6) R23_8 7) 1kb Plus Ladder (Invitrogen) 8) R33C E/N 9) R23_4 S/P 9) R23C_8 S/P]] | ||
- | [[Image:Rb100927_pcrp55andp1.jpg|thumb|350px| | + | [[Image:Rb100927_pcrp55andp1.jpg|thumb|350px|right| Gel 100926-2. PCR results of p55 and p1: p1 with primer 47/48 (lane 1), p1 with primer 55/70 (lane 2), p55 with primer 47/48 (lane3), p55 with primer 55/70]] |
* miniprep of R23C cultures again (colonies from transformation (done [[Igem2010/Main/synthetic miR Kit/September#22/09/2010 | on wednesday]])) | * miniprep of R23C cultures again (colonies from transformation (done [[Igem2010/Main/synthetic miR Kit/September#22/09/2010 | on wednesday]])) | ||
* check of all digestions on an analytical gel (see gel 100926-1) | * check of all digestions on an analytical gel (see gel 100926-1) |
Revision as of 23:23, 26 October 2010
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