Team:Warsaw/Calendar-Stage1/24 July 2010
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(Difference between revisions)
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<br><br>Note: We are not totally satisfied with the results, because of the performance of the cells during the flow cytometry examination, they where adhering to each other. | <br><br>Note: We are not totally satisfied with the results, because of the performance of the cells during the flow cytometry examination, they where adhering to each other. | ||
<br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div> | <br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div> | ||
- | <br> | + | <br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a>) |
<br> | <br> | ||
<br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [Ania P.]</div> | <br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [Ania P.]</div> |
Revision as of 15:23, 27 October 2010
23.07.2010 Friday
Note: We are not totally satisfied with the results, because of the performance of the cells during the flow cytometry examination, they where adhering to each other.
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (RFC 59 standard)
Previous day: Set up liquid cultures of different types of E.coli [Ania P.]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel [Cherry]
legend:
E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)
Data processed with Cell Quest:
Data from Cell Quest processed with Microsoft Excel:
legend:
Hela N- without bacteria
M1,M2,M3,M4- %of events gated with markers M from total
SD- starndard deviation
ERR.-errror
more exact data: