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Team:Alberta/Notebook/protocols/gel extraction
From 2010.igem.org
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Revision as of 01:33, 27 October 2010
Gel Extraction
Reagents:
- Clean scalpel
- Transilluminator
- Eppendorf tube
- Gel extraction kit
- 55oC water bath
- Vortex
- Microfuge
Procedure:
- Place the gel on the transilluminator. Put on face shield and gloves.
- Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
- Pry out the gel ‘cube’ and place it in an eppendorf tube.
- Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
